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WebJan 1, 1992 · This solution of purified antibodies was used in an indirect competitive ELISA for detection of different sulfa drugs (Sheth & Sporns, 1991) using Dynatech Immulon 2 … WebELISA on dynatech "Immulon-2" plates. GSDVHDEL conjugated to BSA was diluted to 50 pmol/ml in NaHCO3 (pH 9.6) and 10 fA0 per well bound to dynatech "Immulon-2" 96-well plates overnight at room temperature. After washing in Tris-buffered saline (TBS) the plates were blocked with 2% non-fat milk in TBS for 10 min at room temperature before use. WebMicrotitre plates with 96-wells (Dynatech Immulon II, Billinghurst, UK) were coated with the conjugate (5 /ig ml"1 in 0.1 M-carbonate-bicarbonate buffer, pH 9.6, 300 /il/well). After overnight incubation at 4°C the plates were washed three times with coating buffer, emptied and dried at 37°C. Finally, the plates hdb first appointment