Myc purification

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August undefined, 2024

WebMyc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification. Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification. 1.

Pierce™ Anti-c-Myc Agarose

WebThe c-Myc peptide tag sequence (EQKLISEEDL) is an epitope that is located within amino acid residues 410-419 of the human c-Myc protein. This tag is used in recombinant protein expression and for purification of c-Myc tagged proteins expressed in bacterial and mammalian cells. Safety Information Storage Class Code 12 - Non Combustible Liquids … Web2 dec. 2024 · The specified purified MYC and C-terminal TS-tagged TRRAP protein constructs were mixed at 50 μM each and incubated at room temperature for 2h in the presence of StrepTactin XT beads in 1X PBS. After pulldown, bound proteins were eluted with 50 mM Biotin and analyzed with a Coomassie-stained SDS-PAGE. brightly colored atlantic fish clue

Myc-tag: An epitope tag for protein characterization, protein

Web‹ Recombinant Protein Purification We offer multiple supports for the efficient purification of DYKDDDDK (FLAG)-, and c-Myc-, HA-tagged proteins using immobilized anti-tag … WebMyc-tag Protein Production & Purification Service Myc-tag (EQKLISEEDL-tag) has been widely used in recombinant protein expression and production. The Myc-tag allows … WebTandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions. The goal is to extract from a cell only the protein … brightly club

DNA binding activities of c-Myc purified from eukaryotic cells

Myc Tag Protein Purification Sino Biological

Webc-Myc Monoclonal Antibody-Agarose Beads may also be used to purify or immunoprecipitate c-Myc-tagged fusion proteins from cell lysates. This product consists … WebA comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. The genomics revolution has created a need for increased … can you get a car towed from your drivewayWeb22 mei 2024 · 3.2 Purification of the c-Myc b-HLH-LZ 1. Take the suspended pellets from −80 °C and immerse in hot tap water (50–60°C). 2. Once the suspension is thawed, freeze the homogenate with liquid nitrogen then thaw in hot water ( see Note 4 ). Repeat this freeze–thaw cycle until the suspension is at maximum viscosity (3–4 cycles). 3. brightly charity jersey

"Web2 dec. 2024 · The specified purified MYC and C-terminal TS-tagged TRRAP protein constructs were mixed at 50 μM each and incubated at room temperature for 2h in the … " - Myc purification

Myc purification

WebA purification protocol was devised which allowed the c-myc protein to be readily purified in quantities sufficient for detailed biochemical and physical analyses. A high-titer polyclonal antiserum was raised against the pure protein and shown to immunoprecipitate the p110gag-myc fusion protein of MC-29-infected quail cells. Web22 aug. 2024 · MYB29, a R2R3-type MYB transcription factor, interacts with MYC3 by its C-terminal MYC-interaction motif. ... For co-purification of WER 67–120/EGL3 1–205, ...

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WebHere, we provide methods to produce and purify high quantities of pure and untagged b-HLH-LZ constructs of c-Myc and Max as well as specific c-Myc/Max heterodimers for their biophysical and structural characterization by CD, NMR, or crystallography. WebThe Myc-Trap provides advantages over conventional IgG antibodies when applied in immunoprecipitation (IP) and affinity purification experiments: no contamination of the …

WebMyc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads. Description. Immunoprecipitation of Myc-tagged proteins and their interacting factors with anti-Myc Nanobody conjugated to beads. • Fast, reliable & efficient one-step immunoprecipitation. WebThe b-HLH-LZ domain of c-Myc is a key target for the development of cancer therapies by blunting its binding to DNA with cell penetrant b-HLH-LZs and/or by stabilizing it into a state that cannot recognize Max to activate and amplify transcription of oncogenic genes.

Web21 jan. 2024 · The anti-c-Myc antibody used to manufacture Anti-c-Myc Agarose is a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The support is crosslinked 4% beaded agarose. This anti-c-Myc affinity resin can be packed … Web25 mei 1992 · The Myc protein is solely responsible for one of these binding activities. Specific sequences that bound to c-Myc were selected from a large pool of random DNA …

Webc-Myc Monoclonal Antibody-Agarose Beads. Monoclonal anti-c-Myc Antibody (Clone 9E10), which recognizes the c-Myc tag. Supplied crosslinked to immobilized protein A agarose beads. May be used to purify or immunoprecipitate c-Myc-tagged fusion proteins from cell lysate. The amount of crosslinked antibody on the agarose beads is >0.5 mg/ml.

Web22 mei 2024 · 3.2 Purification of the c-Myc b-HLH-LZ. 1. Take the suspended pellets from −80 °C and immerse in hot tap water (50–60°C). 2. Once the suspension is thawed, … can you get a car through uberWebMyc-tagged protein purification Monoclonal anti-c- Myc Antibody (Clone 9E10), which recognizes the c-Myc tag (410–419 of the human p62-c-Myc protein), is supplied crosslinked to immobilized Protein A agarose beads. This product may be used to purify or immunoprecipitate c-Myc-tagged fusion proteins from cell lysates. can you get a car with no creditWebRecombinant c-Myc was expressed in a bacterial system (accession number NM 002467) with an N-terminal His-Tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 75% homogeneous and contains no detectable protease, DNase and RNase activity. References brightly cleaningWebMyc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification. Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can … brightly cmmsWebAnti-BPTF (VCU19) from rabbit, purified by affinity chromatography; Synonyms: Nucleosome-remodeling factor subunit BPTF, Bromodomain and PHD finger-containing transcription factor, Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen; find Sigma-Aldrich-ABE1966 MSDS, related peer-reviewed papers, technical documents, similar … can you get a car on pipWebPierce c-Myc peptide may be used to elute c-Myc-tagged proteins from Thermo Scientific Anti-c-Myc Agarose, when the bound protein is sensitive to conditions of chemical elution, such as extremes in pH, or sodium thiocyanate. Related Products Pierce™ Anti-c-Myc Agarose For Research Use Only. Not for use in diagnostic procedures. Specifications Form brightly cleaning servicesWebFeatures of Anti-c-Myc Agarose: • Specific —highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity for immunoprecipitation. • Scalable … can you get a cash advance on flexiti