SpletCurrently, the “gold standard” is real-time reverse transcriptase–polymerase chain reaction (RT-PCR) amplification of the viral DNA for diagnosis of COVID-19 infection. However, RT-PCR may produce initial false-negative results. Long et al suggested that patients with typical CT findings but negative rRT-PCR results should be isolated ... SpletMost tests that detect the ribonucleic acid (RNA) or genetic fingerprint of the virus that causes COVID-19 (e.g., a polymerase chain reaction, or PCR test) use a process where specific bits of the genetic fingerprint are amplified using a temperature cycling reaction that repeats up to 45 times. These are called amplification cycles.
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